Edwardsiella Genus Universal Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0885-ZJF)

Highlights

Pre-mixed reagents simplify operation; only DNA template is required.
Target-specific primers based on conserved gene sequences.
Enhanced detection capability through refined design.

Cat Number: STEM-SLSR-0885-ZJF

Application: For qualitative and quantitative analysis of Edwardsiella Genus Universal

Model: 24351




Description

The kit uses SYBR Green real-time PCR to detect Edwardsiella Genus Universal by amplifying its conserved gene segments. The specific primers and optimized reaction mix enable efficient amplification and signal generation. Fluorescence is captured in real time by the PCR instrument, facilitating sensitive and accurate analysis of the target nucleic acid.

Specification

Kit Components:
Edwardsiella Genus Universal Amplification Mix: 50 µL × 1 tube
Edwardsiella Genus Universal Reaction Mix: 950 µL × 1 tube
Edwardsiella Genus Universal Positive Control: 50 µL × 1 tube
Edwardsiella Genus Universal Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

Pre-mixed reagents simplify operation; only DNA template is required.
Target-specific primers based on conserved gene sequences.
Enhanced detection capability through refined design.
Positive reference material included for result verification.
Closed-tube qPCR process reduces environmental interference.
Intended exclusively for scientific studies.

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