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iPS Cell Line Construction (CAT#: STEM-CBT-0041-WXH)

Introduction

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. The principle is overexpressing four transcription factors in fibroblasts that can make somatic cells return to a state similar to embryonic stem cells. iPS cells have two characteristics of embryonic stem cells: self-renewal ability and multi-directional differentiation potential. The self-renewal ability of iPS cells allows it to be expanded indefinitely, thereby providing unlimited sources of cells; the multi-directional differentiation potential of iPS cells enables it to differentiate into any type of cell in the human body (such as nerve cells, cardiomyocytes, pancreatic cells, liver cells, etc.) , retinal pigment epithelium, red blood platelets and lymphocytes, etc.), can bring an unlimited source of cells to replace those damaged or diseased cells.




Applications

• Disease models: establish cell models of genetic diseases for the development of therapeutic methods.
• Drug development: drug safety and efficacy testing.
• Cell therapy: Differentiate into retinal cells, cardiomyocytes, neuron cells, islet cells, liver cells and other specific functional cells to treat organ.
• Degenerative diseases.
• Regenerative medicine: it can be used to construct functional tissues and organs for repairing defects and for artificial organs.

Procedure

1. Isolation and culture of host cells;
2. Introduce several pluripotency-related genes into host cells through virus-mediated or other means;
3. Plant the virus-infected cells on the feeder cells, and culture them in a special culture system for ES cells, and add corresponding small molecule substances to promote reprogramming as needed during the culture;
4. Identification of iPS cells after the appearance of ES-like clones (cell morphology, epigenetics, in vitro differentiation potential, etc.).

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