Stable Cell Line Construction (CAT#: STEM-CBT-0033-WXH)

Name: Stable Cell Line Construction
SKU: STEM-CBT-0033-WXH
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Introduction

Stable transfection is the integration of exogenous gene DNA into the host cell chromosome so that the host cell can express the target gene and protein for a long time. It is necessary to screen the target cells on the basis of transient transfection or apply a virus with high transfection efficiency and select the appropriate drugs for cell passages according to the resistance markers contained in different gene vectors to obtain cell lines that can stably express the target gene. Stable expression cell lines compensate for the short time of exogenous gene expression in transient infection (or transfection) experiments and facilitate long-term observation.

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Applications Procedure Notes

Applications

• Observation of exogenous gene expression
• long-term pharmacology studies
• Gene therapy research
• Research on the mechanism of genetic regulation
• Large-scale protein synthesis

Procedure

1. Screening of the appropriate antibiotic concentration for the target cells;
2. Suitable virus titer screening for target cells;
3. Lentivirus construction, packaging and titer determination;
4. Lentivirus infection;
5. Screen stable cell lines with specific antibiotics;
6. Identification of stable cell lines.