Investigation of Polyethylenimine (PEI) intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0741-WXH)

Name: Investigation of Polyethylenimine (PEI) intracellular transport by Nanoparticle tracking analysis (NTA)
SKU: STEM-MB-0741-WXH
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Introduction

Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. PEI has a number of uses in laboratory biology, especially tissue culture, but is also toxic to cells if used in excess. Toxicity is by two different mechanisms, the disruption of the cell membrane leading to necrotic cell death (immediate) and disruption of the mitochondrial membrane after internalisation leading to apoptosis (delayed).

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Principle Applications Procedure Materials

Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.