Immobilized Metal Affinity Chromatography Resin NRPB74, ≥40 mg/mL His-tagged protein (CAT#: STEM-AL2-1005-ZJF)

This product is a chromatography medium intended for His-tagged protein purification. It uses 6% cross-linked agarose as the support phase. The functional chemistry is based on iminodiacetic acid. The medium is intended for packed-bed chromatography under the conditions stated in the source data. The source data define the applicable flow, pH, and pressure conditions for the supplied configuration. Chemical compatibility information supports equilibration, washing, regeneration, and cleaning workflows where applicable. Storage conditions are specified to maintain the medium before use and between operations. The product can be integrated into laboratory or process purification workflows consistent with its stated separation mechanism.

Matrix: 6% cross-linked agarose
Ligand: iminodiacetic acid, chelating capacity~30 μmol Ni 2+ /mL
Particle Size Range: 45~165 μm
Average Particle Size: ~90 μm
Dynamic Binding Capacity: ≥40 mg/mL His-tagged protein
Recommended Working Flow Rate: 60~300 cm/h
Maximum Flow Rate And Pressure: 900 cm/h, 0.3 MPa
Operating pH: 4~8.5 (recommended working pH), 3~12 (long-term stability)
2~14 (short-term stability)
Chemical Stability: Stable in common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol
Storage And Transport: 20% ethanol, 2~30°C
Shelf Life: 5 years

Chromatography support matrix
Packed-bed chromatography format
Defined functional ligand chemistry
Selective biomolecule interaction
Prepacked-column configuration available
Defined chemical compatibility
Controlled storage formulation