Ichthyophthirius multifiliis Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1875-ZJF)

Designed for the detection of Ichthyophthirius multifiliis, this kit applies probe-based real-time PCR technology. It uses primers targeting conserved gene sequences of Ichthyophthirius multifiliis, enabling efficient amplification and fluorescent signal generation. Real-time monitoring of fluorescence ensures reliable qualitative and quantitative evaluation of the target nucleic acid.

Kit Components:
Ichthyophthirius multifiliis Amplification Mix: 50 µL × 1 tube
Ichthyophthirius multifiliis Reaction Mix: 950 µL × 1 tube
Ichthyophthirius multifiliis Positive Control: 50 µL × 1 tube
Ichthyophthirius multifiliis Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Simplified operation with pre-mixed reagents requiring only DNA input.
Uses conserved region alignment to ensure high specificity.
Detects low-copy targets with high sensitivity due to optimized chemistry.
Contains a positive control for result validation.
Enclosed one-tube protocol limits contamination.
Fast turnaround for time-sensitive applications.
Restricted to laboratory research applications.

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