Diagnosis of vaginal smears by fluorescence microscope (CAT#: STEM-MIT-0180-LJX)

Introduction

Vaginal smears are part of germinal cytology and are designed to understand ovarian or placental function. For married women, the mucus and cells should be lightly scraped on 1/3 of the lateral wall of the vagina to avoid mixing deep cells and affecting the diagnosis. The thin and even smear and slide should be fixed in 95% ethanol.

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Principle Applications Procedure Materials Notes Related Products

Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Vaginal discharge

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye

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