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Studies on neurosecretion in the river lamprey by fluorescence microscope (CAT#: STEM-MIT-0182-LJX)

Introduction

Lampreys belong to the family Lampreys of the order Lampreys.<br />It is found in northeast China, Europe, northern Asia, Korea, Japan and North America. Its body is slender, eel-shaped, and its skin is bare and scaleless; The eye is sensitive to light; The inner ear has two semicircular canals, which are bisexualism. Its early habitat in the sea, as they grow up to swim to freshwater rivers to spawn.




Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Nerve cells of lamprey

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye