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Determining the available phosphorus in soil by spectrophotometry using bromophenol blue as acid-base indicator (CAT#: STEM-PPA-0028-LJX)

Introduction

The standard method and general method for determining available phosphorus in soil are usually used to extract samples with acid or alkali solution, adjust the pH value of the extract solution, and then determine by spectrophotometry. The filtration operation of this method is complicated, and the color of acid-base indicator dinitrophenol is easily disturbed by the background color of solution and is toxic. This service adopts centrifugal separation instead of filtration, and uses bromophenol blue as an indicator to adjust pH. The color mutation is more obvious, which is not easily affected by the background color of the liquid to be tested, and has low toxicity and accurate test data.




Principle

Acid-base indicators produce different colors in aqueous solutions of their specific pH range. The indicator is added to the standard buffer solution, and the resulting color is used as the standard colorimetric tube. The pH value of the water sample can be measured by visual colorimetric comparison with the water sample colorimetric tube with the same indicator.

Applications

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

Procedure

1. Add ph indicator to the standard solution, and the solution with different pH will show different colors. Use these solutions as standard colorimetric tubes
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution

Materials

• Sample Type:
Soil

Notes

After dropping the indicator into the solution, it should be gently shaken to avoid inaccurate detection.