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Determining the serum total protein by bromocresol green - ethanol method (CAT#: STEM-PPA-0035-LJX)

Introduction

Bromocresol green (BCG) is a phenolsulfonyl peptide pH indicator, which has been widely used in albumin determination due to its "protein error". Reducing the pH of BCG reagent and adding proper amount of methanol can obviously improve the binding force of pigment to globulin amino acid residues for the determination of serum total protein.




Principle

Acid-base indicators produce different colors in aqueous solutions of their specific pH range. The indicator is added to the standard buffer solution, and the resulting color is used as the standard colorimetric tube. The pH value of the water sample can be measured by visual colorimetric comparison with the water sample colorimetric tube with the same indicator.

Applications

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

Procedure

1. Add ph indicator to the standard solution, and the solution with different pH will show different colors. Use these solutions as standard colorimetric tubes
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution

Materials

• Sample Type:
Serum

Notes

After dropping the indicator into the solution, it should be gently shaken to avoid inaccurate detection.