This weak anion-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses high-flow agarose functionalized with diethylaminoethyl groups. The weak-base ligand provides pH-dependent anion-exchange selectivity. The 24-44 µm particles support efficient mass transfer and high chromatographic resolution. A BSA adsorption capacity above 50 mg/mL supports preparative purification. The medium operates above 150 cm/h under the stated test conditions. Low nonspecific adsorption contributes to product recovery and process consistency. Broad chemical compatibility and steam resistance support repeated cleaning and reuse.
Specification
Model: DEAE Seplife HP Appearance: White spherical gel, odorless and tasteless Type: Weak anion-exchange medium Matrix: Seplife 6FF Ligand: Diethylaminoethyl Particle size: 24-44 µm BSA adsorption capacity:>50 mg/mL Ion-exchange capacity: 0.11-0.16 mmol/mL Maximum flow rate:>150 cm/h Pressure resistance: 0.3 MPa Operating temperature: 4-40°C Long-term pH stability: 2-12 Short-term CIP pH stability: 1-14 Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol Heat resistance: 121°C in water for 30 min
Features
High-resolution fine particles Weak DEAE selectivity >50 mg/mL BSA capacity Broad CIP pH range Low nonspecific adsorption Strong chemical stability Steam-resistant agarose Suitable for polishing purification