rProtein A Seplife Suni Antibody Affinity Medium, 50 mg/mL (CAT#: STEM-BC-3146-ZJF)

This Protein A affinity medium is designed for capture and purification of monoclonal and polyclonal antibodies. It uses highly crosslinked spherical agarose microspheres. An alkali-resistant recombinant Protein A ligand is coupled to the matrix. The ligand selectively binds the Fc region of compatible antibodies. A single affinity step can purify antibodies from ascites, serum, or cell-culture fluid. The medium provides a stated binding capacity of 50 mg/mL. High-flow performance supports efficient processing at laboratory and production scales. Alkali resistance allows cleaning with sodium hydroxide between purification cycles.

Model: rProtein A Seplife Suni
Appearance: Spherical gel, odorless and tasteless
Matrix: Highly crosslinked agarose microspheres
Ligand: Alkali-resistant recombinant Protein A
Median particle size d50: Approximately 90 µm
Flow rate:>300 cm/h at 0.1 MPa
Pressure resistance: 0.3 MPa
Binding capacity: 50 mg/mL
Ligand density: 5-6 mg/mL
Alkali resistance: 0.1-0.5 M sodium hydroxide
Example purification result: 45.5 mg/mL loading, 95.9% SEC purity, and 99.1% recovery
Example CIP: 0.5 M sodium hydroxide

Specific antibody Fc binding
50 mg/mL capacity
Alkali-resistant ligand
High-flow agarose matrix
One-step antibody capture
Suitable for varied feedstocks
High example recovery
Reusable after alkaline cleaning

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