DEAE Seplife A50 Weak Anion-Exchange Medium, 110 mg Albumin/mL (CAT#: STEM-BC-3172-ZJF)

This weak anion-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses highly crosslinked dextran G50 as the chromatographic matrix. Diethylaminoethyl groups provide pH-dependent anion-exchange selectivity. The medium is supplied in the chloride form. Dry particles range from 40 to 120 µm. An albumin adsorption capacity of 110 mg/mL supports preparative downstream processing. High flow and relatively low backpressure facilitate scale-up. The medium is stable in common aqueous buffers, urea, and guanidine hydrochloride. It also tolerates the stated steam-treatment conditions in sodium chloride solution.

Model: DEAE Seplife A50
Appearance: White powder, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: Dextran G50
Ligand: Diethylaminoethyl
Ionic form: Chloride
Dry particle size: 40-120 µm
Albumin adsorption capacity: 110 mg/mL
Ion-exchange capacity: 3.0-4.0 mmol/g dry gel
Working pH range: 2-9
Long-term pH stability: 2-12
Short-term pH stability: 2-12
Chemical compatibility: Common aqueous buffers, 8 M urea, and 6 M guanidine hydrochloride
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min

Weak DEAE selectivity
110 mg/mL albumin capacity
High ion-exchange capacity
Crosslinked dextran G50 matrix
Low operating backpressure
Denaturant-compatible medium
Steam-treatment tolerance
Suitable for scale-up

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