Butyl Seplife 4B Hydrophobic Interaction Medium,>26 mg BSA/mL (CAT#: STEM-BC-3137-ZJF)

This hydrophobic interaction medium is designed for purification of hepatitis B vaccine components. It uses a four-percent agarose gel matrix functionalized with butyl ligands. Target molecules bind through hydrophobic interactions under suitable high-salt conditions. Reducing ionic strength releases bound components according to their hydrophobicity. The spherical particles provide uniform packing and stable separation performance. Protein adsorption above 26 mg BSA/mL supports preparative purification. The medium operates at 70-140 cm/h under the specified pressure condition. Broad pH stability allows repeated cleaning-in-place. The matrix can withstand the stated steam-treatment conditions in sodium chloride solution.

Model: Butyl Seplife 4B
Appearance: White spherical gel
Matrix: 4% agarose gel
Ligand: Butyl
Shape: Spherical
Particle size: 45-165 µm
Pressure-flow range: 70-140 cm/h at 0.15 MPa
Protein adsorption capacity:>26 mg BSA/mL
Operating temperature: 4-40°C
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 50% ethylene glycol, 8 M urea, 6 mol/L guanidine hydrochloride, and 1 mM hydrochloric acid
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min

Hepatitis B vaccine application
Butyl interaction chemistry
>26 mg/mL BSA capacity
Broad chemical stability
Steam-tolerant matrix
Wide CIP pH range
Uniform agarose particles
Mild hydrophobic purification

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