CM Seplife FF Weak Cation-Exchange Medium, 60 mg Lysozyme/mL (CAT#: STEM-BC-3168-ZJF)

This weak cation-exchange medium is designed for downstream purification of proteins, nucleic acids, and peptides. It uses high-flow agarose functionalized with carboxymethyl groups. The weak-acid ligand provides selective binding controlled by process pH and ionic strength. The 45-165 µm particles support high permeability and relatively low backpressure. A lysozyme adsorption capacity of 60 mg/mL supports preparative capture and intermediate purification. The medium provides a maximum stated flow rate of 750 cm/h. Broad chemical compatibility supports alkaline cleaning and exposure to denaturants and ethanol. The matrix withstands the specified steam-treatment conditions for repeated process use.

Model: CM Seplife FF
Appearance: White spherical gel, odorless and tasteless
Type: Weak cation-exchange medium
Matrix: Seplife 6FF
Ligand: Carboxymethyl
Particle size: 45-165 µm
Lysozyme adsorption capacity: 60 mg/mL
Ion-exchange capacity: 0.09-0.13 mmol/mL
Maximum flow rate: 750 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-30°C
Long-term pH stability: 4-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

Weak carboxymethyl selectivity
60 mg/mL lysozyme capacity
Up to 750 cm/h flow
Low operating backpressure
Broad chemical compatibility
Wide CIP pH tolerance
Steam-resistant agarose
Suitable for scalable purification

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