SP Seplife FF Strong Cation-Exchange Medium,>55 mg Lysozyme/mL (CAT#: STEM-BC-3081-ZJF)

This strong cation-exchange chromatography medium is designed for downstream purification of proteins, nucleic acids, and peptides. It uses a highly crosslinked agarose matrix functionalized with sulfopropyl groups. The spherical gel particles support high flow with relatively low backpressure. A lysozyme adsorption capacity above 55 mg/mL supports productive capture and intermediate purification. Low nonspecific adsorption helps maintain product recovery during chromatographic processing. The matrix provides chemical and mechanical stability for repeated process use. Its broad short-term pH range supports cleaning-in-place procedures. The medium can be scaled from process development to manufacturing columns. It also tolerates the stated steam-treatment conditions for compatible applications.

Model: SP Seplife FF
Appearance: White spherical gel, odorless and tasteless
Type: Strong cation-exchange medium
Matrix: Seplife 6FF
Ligand: Sulfopropyl group
Particle size: 50-160 µm
Long-term pH stability: 4-13
Short-term CIP pH stability: 3-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Adsorption capacity:>55 mg lysozyme/mL
Ion-exchange capacity: 0.18-0.25 mmol/mL
Operating temperature: 4-40°C
Heat resistance: 121°C in water for 30 min
Maximum flow rate: 750 cm/h
Pressure resistance: 0.3 MPa
Test conditions: 15 cm bed height, 25°C, and 0.1 mol/L sodium chloride

Strong sulfopropyl cation exchange
High lysozyme capacity
Up to 750 cm/h flow
Low operating backpressure
Broad CIP pH range
Strong chemical stability
Low nonspecific adsorption
Scalable downstream processing

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