Seplife LXPM-Ni5504 IDA C Affinity Medium, 800-1000 cm/h (CAT#: STEM-BC-3125-ZJF)

This high-flow immobilized-metal affinity medium is designed for capture and purification of His-tagged proteins. It uses large polymethyl methacrylate particles with a hydrophilically modified surface. IDA chelating groups coordinate nickel ions for selective histidine binding. The 200 µm particle size provides high permeability and low operating resistance. A 1000 Å pore structure supports access by proteins and other compatible biomolecules. The medium provides a stated His-protein capacity of at least 18 mg/mL. Nickel-ion binding of approximately 55 µmol/mL supports stable affinity functionality. Its rigid particles allow linear flow from 800 to 1000 cm/h with a low compression factor.

Model: Seplife® LXPM-Ni5504 IDA C
Matrix: Polymethyl methacrylate
Appearance: Light-blue opaque spherical particles
Chelating chemistry: IDA with Ni²⁺
Median particle size d50v: 200 µm
Pore size: 1000 Å
His-tagged protein capacity: ≥18 mg/mL
Nickel-ion binding: Approximately 55 µmol Ni²⁺/mL
Pressure-flow range: 800-1000 cm/h at 22 mm × 20 cm bed and 3 bar
Compression factor: ≤1.05
Maximum pressure: 0.5 MPa
pH stability: 2-13
Storage solution: 20% ethanol with stated sodium chloride preservative
Chemical compatibility: 0.1 M sodium hydroxide, 8 M urea for 24 h, 6 M guanidine hydrochloride for 24 h, 1 M sodium hydroxide for 24 h, and 5 mM DTT for 24 h

Very high linear flow
Large 200 µm particles
IDA nickel affinity chemistry
Low compression factor
≥18 mg/mL His-protein capacity
High nickel-ion content
Broad pH stability
Suitable for rapid capture

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