Bursaphelenchus Xylophilus Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1051-ZJF)

Optimized for high-throughput and research applications, this kit uses SYBR Green dye-based real-time PCR for the detection of Bursaphelenchus Xylophilus. Amplification of the target gene leads to fluorescence emission, which is monitored in real time. This allows for effective and reproducible detection of target nucleic acids across a range of experimental conditions.

Kit Components:
Bursaphelenchus Xylophilus Amplification Mix: 50 µL × 1 tube
Bursaphelenchus Xylophilus Reaction Mix: 950 µL × 1 tube
Bursaphelenchus Xylophilus Positive Control: 50 µL × 1 tube
Bursaphelenchus Xylophilus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Designed for immediate use with user-provided DNA.
Selective primers crafted from highly conserved genetic regions.
Fine-tuned probe and primer chemistry enhances detection power.
Includes control sample for clear interpretation.
Sealed fluorescence PCR tube reduces contamination possibilities.
For investigational use in laboratories.

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STEM-SLSR-1050-ZJF
STEM-SLSR-1052-ZJF
STEM-SLSR-1053-ZJF