Milker’s Nodule Virus Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0586-ZJF)

This kit offers a sensitive and specific method for detecting Milker’s Nodule Virus using SYBR Green-based real-time PCR. Designed with primers targeting conserved regions, the assay produces fluorescence signals correlating with amplification levels. The real-time monitoring capability ensures accurate qualitative and quantitative results.

Kit Components:
Milker’s Nodule Virus Amplification Mix: 50 µL × 1 tube
Milker’s Nodule Virus Reaction Mix: 950 µL × 1 tube
Milker’s Nodule Virus Positive Control: 50 µL × 1 tube
Milker’s Nodule Virus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring minimal preparation—just input the DNA.
High accuracy through primers tailored to conserved regions.
Excellent assay sensitivity via optimized components.
Positive control provided to ensure analytical reliability.
Fully enclosed fluorescence PCR reaction prevents cross-contamination.
For laboratory research purposes only.

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STEM-SLSR-0587-ZJF
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