This boronic-acid affinity medium is designed for separation of compounds containing cis-diol groups. It uses a polymethyl methacrylate matrix functionalized with a boronic-acid ligand. Reversible interaction between boronic acid and cis-diols enables adsorption and desorption by changing the pH. The medium is suitable for glycoproteins, carbohydrates, nucleosides, nucleic acids, peptides, and enzymes. The hydrophilic polymeric matrix supports low nonspecific adsorption. An 80 µm particle size and 1000 Å pores support preparative biomolecule processing. The rigid medium operates at flow rates from 100 to 800 cm/h and pressures up to 0.8 MPa. The resin can be cleaned with the stated alkaline solution and stored in aqueous ethanol.
Specification
Model: Boricacid Seplife® LXPM5504 Matrix: Polymethyl methacrylate Ligand: Boronic-acid compound Particle size: 80 µm Pore size: 1000 Å Ligand-density value stated: 20, with source unit recorded as mmol/mL Binding-capacity value: Not reliably legible in the supplied source Maximum pressure: 0.8 MPa Recommended flow: 100-800 cm/h pH stability: 2-12 CIP cleaning: Stated as 0.1-0.5 M solution Storage: 20% ethanol at approximately 2-8°C
Features
Selective cis-diol interaction Reversible pH-controlled binding Suitable for glycoproteins and sugars Rigid 0.8 MPa matrix 1000 Å pore structure Up to 800 cm/h flow Hydrophilic low-binding surface Affinity purification versatility