Boricacid Seplife LXPM5504 Affinity Medium, 80 µm (CAT#: STEM-BC-3128-ZJF)

Highlights

Selective cis-diol interaction
Reversible pH-controlled binding
Suitable for glycoproteins and sugars

Cat Number: STEM-BC-3128-ZJF

Application: for cis-diol biomolecule affinity purification

Model: Boricacid Seplife® LXPM5504




Description

This boronic-acid affinity medium is designed for separation of compounds containing cis-diol groups. It uses a polymethyl methacrylate matrix functionalized with a boronic-acid ligand. Reversible interaction between boronic acid and cis-diols enables adsorption and desorption by changing the pH. The medium is suitable for glycoproteins, carbohydrates, nucleosides, nucleic acids, peptides, and enzymes. The hydrophilic polymeric matrix supports low nonspecific adsorption. An 80 µm particle size and 1000 Å pores support preparative biomolecule processing. The rigid medium operates at flow rates from 100 to 800 cm/h and pressures up to 0.8 MPa. The resin can be cleaned with the stated alkaline solution and stored in aqueous ethanol.

Specification

Model: Boricacid Seplife® LXPM5504
Matrix: Polymethyl methacrylate
Ligand: Boronic-acid compound
Particle size: 80 µm
Pore size: 1000 Å
Ligand-density value stated: 20, with source unit recorded as mmol/mL
Binding-capacity value: Not reliably legible in the supplied source
Maximum pressure: 0.8 MPa
Recommended flow: 100-800 cm/h
pH stability: 2-12
CIP cleaning: Stated as 0.1-0.5 M solution
Storage: 20% ethanol at approximately 2-8°C

Features

Selective cis-diol interaction
Reversible pH-controlled binding
Suitable for glycoproteins and sugars
Rigid 0.8 MPa matrix
1000 Å pore structure
Up to 800 cm/h flow
Hydrophilic low-binding surface
Affinity purification versatility

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