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Antibody Purification by NHS-Activated Magnetic Beads (CAT#: STEM-MB-1309-LGZ)
Introduction
Magnetic bead purification technology has significant advantages over traditional protein and nucleic acid purification techniques. Rapid purification of proteins and antibodies is simple with coated magnetic beads. Save time and money by replacing nucleic acid-based purification columns with silica-coated magnetic bead technology.
Principle
NHS-Activated MagBeads are pre-activated polymer magnetic microspheres, the surface of which is modified with NHS groups, which can be coupled with amino-containing proteins or polypeptides, and are mainly used for affinity purification of proteins/antibodies.
Applications
For the purification of antibody.
Procedure
1. Buffer preparation.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.
Materials
• Washing Buffer
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet
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Wide range of applications
For various laboratory needs
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