Animal Model of Chronic Rejection in Heart Transplantation (CAT#: STEM-AE-0026-WXH)

• To study the mechanism and surgical treatment of heart transplantation.
• To study the mechanism and preventive measures of chronic rejection after heart transplantation.
• Drug development, drug screening and efficacy research.

1. Anesthesia and disinfection.
2. Make a midline abdominal incision, open the chest cavity, and fill the heart with saline ice cubes to stop the beating.
3. Ligating the superior and inferior vena cava of the heart, and disconnecting the aortopulmonary artery.
4. Ligate the pulmonary veins, cut off all blood vessels, and free the heart.
5. Recipient preparation: Anesthesia and disinfection.
6. Abdominal median incision, free abdominal aorta and inferior vena cava.
7. Take out the donor heart and place it in the recipient's abdominal cavity, align the aorta with the abdominal aorta, align the pulmonary artery with the inferior vena cava, and suture.
8. Loosen the ligature, the heart turns red, and then resumes beating.
9. Close the abdominal cavity, place the mouse in a warm environment, and wait for it to wake up naturally.