Anaplasma Centrale Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1175-ZJF)

Optimized for high-throughput and research applications, this kit uses SYBR Green dye-based real-time PCR for the detection of Anaplasma Centrale. Amplification of the target gene leads to fluorescence emission, which is monitored in real time. This allows for effective and reproducible detection of target nucleic acids across a range of experimental conditions.

Kit Components:
Anaplasma Centrale Amplification Mix: 50 µL × 1 tube
Anaplasma Centrale Reaction Mix: 950 µL × 1 tube
Anaplasma Centrale Positive Control: 50 µL × 1 tube
Anaplasma Centrale Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Pre-mixed reagents simplify operation; only DNA template is required.
Target-specific primers based on conserved gene sequences.
Enhanced detection capability through refined design.
Positive reference material included for result verification.
Closed-tube qPCR process reduces environmental interference.
Intended exclusively for scientific studies.

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