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Analysis of Uromodulin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0618-LGZ)
Introduction
Uromodulin (also known as Tamam-Horsfall glycoprotein or THP) is a urinary glycoprotein of 85 - 95 kDa. It is secreted by the renal tubular epithelium, acts as a binding protein for IL-1, TNF-α, and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the renal tubules.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Immunology/Inflammation, Kidney Injury, Toxicology
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Plasma, Serum
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