Analysis of Prolactinomas by RT-qPCR (CAT#: STEM-MT-0003-LGZ)

Name: Analysis of Prolactinomas by RT-qPCR
SKU: STEM-MT-0003-LGZ
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Introduction

The molecular pathogenesis of prolactinomas has not been elucidated; no mutational changes were found except for RAS mutations in a single aggressive prolactinoma. In prolactinomas, another obstacle is the lack of surgical specimens suitable for molecular analysis, as prolactinomas are rarely resected due to the availability and effectiveness of drug therapy. Changes in gene expression have been sought and detected in the absence of mutational events.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Prolactinomas

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.