Analysis of HTRA4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1877-LGZ)

Name: Analysis of HTRA4 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1877-LGZ
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Introduction

Official Full Name: HtrA serine peptidase 4
This gene encodes a member of the HtrA protease family. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies by other related family members, this enzyme may be a secreted oligomeric chaperone protease that degrades misfolded secreted proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis and aging.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.