Analysis of NOC2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1352-LGZ)

Name: Analysis of NOC2L Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1352-LGZ
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Introduction

Official Full Name: NOC2 like nucleolar associated transcriptional repressor
Also known as: NIR; NET7; NET15; PPP1R112
Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT).

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.