Analysis of NME7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0483-LGZ)

Name: Analysis of NME7 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0483-LGZ
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Introduction

Official Full Name: NME/NM23 family member 7
Also known as: NDK7; NDK 7; CFAP67; MN23H7; nm23-H7
This gene encodes a member of the non-metastatically expressed nucleoside diphosphate kinase family. Members of this family are enzymes that catalyze the transfer of phosphate from nucleoside triphosphates to nucleoside diphosphates. The protein contains two kinase domains, one of which is involved in autophosphorylation and the other may be inactive. The protein is localized to the centrosome and functions in microtubule organization as part of the γ-tubulin ring complex. Mutations in this gene may be associated with venous thromboembolism. Alternate splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.