Analysis of ATAD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2058-LGZ)

Name: Analysis of ATAD1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2058-LGZ
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Introduction

Official Full Name: ATPase family AAA domain containing 1
Also known as: Msp1; AFDC1; HKPX4; FNP001; hATAD1; THORASE
Predicted to enable ATP binding activity and transmembrane protein dislocase activity. Involved in extraction of mislocalized protein from mitochondrial outer membrane. Located in mitochondrial outer membrane and peroxisomal membrane. Implicated in hyperekplexia 4.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.