Analysis of LOC107305685 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1461-LGZ)

Name: Analysis of LOC107305685 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1461-LGZ
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Introduction

Gene description: 2q12.3 distal recombination region
This region has been shown to undergo non-allelic homologous recombination (NAHR) with a similar low-copy repeat region, the 2q13 recombination region, located about 1.3 Mb downstream of this region, in direct orientation on the reference genome. Deletion of the intervening sequence as a result of NAHR between these recombination regions has been observed. This region overlaps an Alu element.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.