Analysis of TUBB4B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1978-LGZ)

Name: Analysis of TUBB4B Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1978-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: tubulin beta 4B class IVb
Also known as: Beta2; TUBB2; LCAEOD; TUBB2C
Enables double-stranded RNA binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Located in microtubule. Implicated in Leber congenital amaurosis with early-onset deafness.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.