Analysis of Factor VII (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0165-LGZ)

Introduction

Factor VII (proconvertin) is one of the proteins in the coagulation cascade that causes blood to coagulate. It is an enzyme of the serine protease class. Once bound to tissue factor released from damaged tissue, it is converted to factor VIIa (or factor VIIa, activated factor VII), which in turn activates factors IX and X. The action of this factor is blocked by tissue factor pathway inhibitor (TFPI), which is released almost immediately after blood clotting begins.

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Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Goat Polyclonal
Detection Antibody: Goat Polyclonal

Applications

Kidney Injury, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Cell Culture Media, Plasma, Serum
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