Analysis of Equine Herpesvirus (EHV) by Real-Time PCR Method (CAT#: STEM-MB-3173-LGZ)

Introduction

Equine herpesviruses are very common DNA viruses in horse populations worldwide. The two most significant are EHV-1, which causes respiratory disease, abortion, and neurologic disease; and EHV-4, which primarily causes respiratory disease and only occasionally causes abortion or neurologic disease. Fatal neonatal disease infrequently occurs as a result of in utero infection. Asymptomatic infection is common and facilitates transmission within groups of horses.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.