Analysis of AP2A2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2196-LGZ)

Name: Analysis of AP2A2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2196-LGZ
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Introduction

Official Full Name: adaptor related protein complex 2 subunit alpha 2
Also known as: HIP9; HYPJ; ADTAB; HIP-9; CLAPA2
The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.