Analysis of GNPAT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1013-LGZ)

Name: Analysis of GNPAT Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1013-LGZ
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Introduction

Official Full Name: glyceronephosphate O-acyltransferase
Also known as: DAPAT; RCDP2; DAP-AT; DHAPAT
This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.