Analysis of ADAM12 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0010-LGZ)

Introduction

A Disintegrin And Metalloproteinases(ADAMs) family is a class of zinc ion-dependent transmembrane glycoprotein hydrolases containing several domains, such as propeptide domain, metalloproteinase domain, deintegrin domain, cysteine-rich domain, and epithelial growth factor-like domain near the membrane. They are involved in a variety of biological processes, including proteolysis, signal transduction, cell adhesion, migration, etc., and are closely related to the occurrence and invasion of tumors.

STEMart technology detail



Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Reproductive Biology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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