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Analysis of Respiratory Syncytial Virus Pre-Fusion F (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0526-LGZ)

Introduction

Respiratory syncytial virus is the leading viral cause of pneumonia and bronchitis in infants and young children. F (Fusion) protein is one of the envelope proteins of respiratory syncytial virus, which plays a role in membrane fusion during virus infection and is the most promising respiratory syncytial virus antigen. It exists in two conformations, pre-fusion and post-fusion.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Respiratory

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Saliva, Upper Respiratory Sample
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