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Analysis of AChE (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0008-LGZ)

Introduction

Acetylcholinesterase (AchE) is a key enzyme in biological nerve conduction. In the cholinergic synapses, ACHE can degrade acetylcholine, stop the excitatory effect of neurotransmitters on the postsynaptic membrane, and ensure the normal transmission of nerve signals in the biological body. It has the activity of carboxypeptidase and aminopeptidase. Acetylcholinesterase is involved in cell development and maturation, and can promote neuronal development and nerve regeneration.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum