Analysis of cIAP2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0107-LGZ)

Introduction

The inhibitor of apoptosis protein (IAP) family comprises a group of evolutionarily conserved inhibitors of apoptosis that contain a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Human protein members of this family include c-IAP1, c-IAP2, XIAP, surviving, livin, and NAIP. Members of the IAP family are overexpressed in cancer cell lines and primary tumors, and these proteins play an important role in cancer progression.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma

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