Analysis of ABCC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2471-LGZ)

Name: Analysis of ABCC1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2471-LGZ
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Introduction

Official Full Name: ATP binding cassette subfamily C member 1
Also known as: MRP; ABCC; GS-X; MRP1; ABC29; DFNA77
The protein encoded by this gene is a member of the ATP-binding cassette transporter superfamily. ABC proteins transport various molecules outside and inside the cell membrane. The ABC genes are grouped into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily and is involved in multidrug resistance. The protein acts as a multispecific organic anion transporter with oxidized glutamate, cysteine leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronate and sulfate conjugates of steroid hormones and bile salts. Alternative splicing variants of this gene have been described, but the full-length nature is unknown.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.