Analysis of DARS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0898-LGZ)

Name: Analysis of DARS2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0898-LGZ
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Introduction

Official Full Name: aspartyl-tRNA synthetase 2, mitochondrial
Also known as: LBSL; ASPRS; mtAspRS; MT-ASPRS
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.