Toll-Like Receptor 2 (TLR2) Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0217-ZJF)

Highlights

No setup required—add DNA and begin.
Superior specificity derived from conserved sequence primer design.
Designed for heightened assay sensitivity.

Cat Number: STEM-SLSR-0217-ZJF

Application: For qualitative and quantitative analysis of Toll-Like Receptor 2 (TLR2)

Model: 25032




Description

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Toll-Like Receptor 2 (TLR2) nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Toll-Like Receptor 2 (TLR2) in experimental and routine laboratory settings.

Specification

Kit Components:
Toll-Like Receptor 2 (TLR2) Amplification Mix: 50 µL × 1 tube
Toll-Like Receptor 2 (TLR2) Reaction Mix: 950 µL × 1 tube
Toll-Like Receptor 2 (TLR2) Positive Control: 50 µL × 1 tube
Toll-Like Receptor 2 (TLR2) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

No setup required—add DNA and begin.
Superior specificity derived from conserved sequence primer design.
Designed for heightened assay sensitivity.
Positive control ensures detection integrity.
One-tube closed system improves contamination control.
Use limited to non-clinical research.

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