Taylorella equigenitalis Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1440-ZJF)

Highlights

All reagents pre-prepared; template DNA addition is the only step.
Probes and primers designed for precise recognition of target sequence.
High-sensitivity detection capability built into the system.

Cat Number: STEM-SLSR-1440-ZJF

Application: For qualitative and quantitative analysis of Taylorella equigenitalis

Model: 22546

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Description

Using high-performance probes and precision-designed primers, this real-time PCR kit enables the detection of Taylorella equigenitalis by targeting conserved genomic regions. Fluorescence signals are emitted as the target is amplified, and the system records data in real time for confident qualitative and quantitative evaluation.

Specification

Kit Components:
Taylorella equigenitalis Amplification Mix: 50 µL × 1 tube
Taylorella equigenitalis Reaction Mix: 950 µL × 1 tube
Taylorella equigenitalis Positive Control: 50 µL × 1 tube
Taylorella equigenitalis Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Features

All reagents pre-prepared; template DNA addition is the only step.
Probes and primers designed for precise recognition of target sequence.
High-sensitivity detection capability built into the system.
Includes positive control for experimental accuracy.
Closed-tube design reduces the chance of contamination.
Accelerated protocol supports time-saving diagnostics.
Only for use in research environments.

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