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Structural analysis of Papain by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1084-WXH)
Introduction
Papain is an enzyme found in the white fluid (latex) that occurs in raw papaya fruit. It is a protease, meaning it breaks down proteins. Papain contains substances that might help fight infection and heal wounds.
People use papain for dental cavities, shingles, parasite infections, jellyfish stings, wound healing, and many other conditions, but there is no good scientific evidence to support these uses.
Principle
Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a branch of magnetic resonance spectroscopy which utilizes microwave radiation to probe species with unpaired electrons, such as radicals, radical cations, and triplets in the presence of an externally applied static magnetic field.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.
Applications
• Study dynamic organisation of lipids in biological membranes, lipid-protein interactions and temperature of transition of gel to liquid crystalline phases.
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine
Procedure
1. Sample Preparation
2. Electron paramagnetic resonance (EPR) spectroscopy testing
48. Data analysis
2. Electron paramagnetic resonance (EPR) spectroscopy testing
48. Data analysis
Materials
• EPR Spectrometer
• Spectrophotometer
• Spectrophotometer
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