Structural analysis of Monoclonal Antibody by Circular dichroism (CD) (CAT#: STEM-MB-0631-WXH)

Name: Structural analysis of Monoclonal Antibody by Circular dichroism (CD)
SKU: STEM-MB-0631-WXH
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Introduction

Monoclonal antibodies are laboratory-produced molecules engineered to serve as substitute antibodies that can restore, enhance, modify or mimic the immune system's attack on cells that aren't wanted, such as cancer cells. Briefly, mAbs are comprised of four polypeptide chains, two identical heavy chains and two identical light chains, with a total molecular weight of ~150kD. The chains are held together by disulfide linkages and fold to form a “Y” shaped tetramer

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Principle Applications Procedure Materials

Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.