Stain mouse brain tissue by Golgi-Cox silver staining technology (CAT#: STEM-CT-0008-LJX)

The solution of potassium dichromate soaked in brain tissue reacts with the solution of silver nitrate to form a black precipitate of silver chromate, which is deposited in nerve cells due to the argyrophilic nature of the tissue.

Staining of nerve tissue section samples from experimental animals

1.Rat perfusion fixation
2.The whole rat brain was placed in a wide brown bottle filled with Golgi-Cox dye
3.After sealing the bottle, place it in an anti-light container, and then place it in a 37℃ incubator for dip-staining.
4.After staining, remove the specimen and wash it with pure water for 3 times
5.Slice
6.Color with ammonia
7.Wash with pure water
8.Attach the section to the clean slide
9.Xylene transparent
10.Seal the slices

• Sample Type:
Mouse brain tissue

1.The dye solution should be prepared for use and stored away from light.
2.The surface and bottom of the dye solution are prone to sediment, so it must be filtered before use.
3.During the slicing process, try to flatten the slices as much as possible to prevent them from breaking during the sealing process.