SP Seplife BB Strong Cation-Exchange Medium, 1200-1800 cm/h (CAT#: STEM-BC-3078-ZJF)

This strong cation-exchange medium is designed for downstream purification of proteins, nucleic acids, and peptides. It uses six-percent crosslinked agarose functionalized with sulfonic-acid groups. The large spherical particles provide high permeability and low operating backpressure. Strong cation-exchange functionality remains ionized across a broad working range. The medium supports high linear flow under the specified pressure differential. Broad chemical compatibility allows use with common buffers, denaturants, alcohols, and cleaning solutions. Low nonspecific adsorption supports good product recovery. The medium can be cleaned in place and reused in scalable purification processes. It is stored in an ethanol and sodium acetate solution at controlled temperature.

Model: SP Seplife BB
Appearance: White spherical gel, odorless and tasteless
Type: Strong cation-exchange medium
Matrix: 6% crosslinked agarose
Ligand: Sulfonic-acid group
Median particle size d50: 200 µm
Particle-size range: 100-300 µm
Long-term pH stability: 4-12
Short-term CIP pH stability: 3-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, 70% ethanol, and 30% isopropanol
Ion-exchange capacity: 0.18-0.25 mmol H+/mL
Operating temperature: 4-30°C
Heat resistance: 121°C in water for 30 min
Flow rate: 1200-1800 cm/h at 0.1 MPa differential pressure and 25 cm bed height
Storage: 4-30°C in 20% ethanol containing 0.2 M sodium acetate

Strong cation-exchange chemistry
Very high linear flow
Large permeable particles
Low backpressure
Broad chemical compatibility
CIP-compatible medium
Steam-resistant agarose matrix
Scalable biomolecule purification

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