Shinetek CELLine 350 Bioreactor, Shinetek Instruments Research Institute (CAT#: STEM-CC-3263-LGZ)

Advanced cell culture device, designed membrane culture flask, suitable for culturing antibody and protein products.
Newly launched high-density cell culture device. This design will greatly increase the density of the product when culturing antibody and protein products on a small scale. Conventional methods of culturing cells in vivo or in vitro are difficult, often yield very low cell densities and require efficient purification methods to obtain the product. CELLine bioreactors address these three limitations when growing cells in static flasks.
Wheaton's CELLine bioreactor adopts the semipermeable membrane separation culture technology of the cell chamber and the medium chamber, breaking through the constraints of air, nutrients, and metabolic inhibitors on cell growth in traditional cell culture. To a large extent, it simulates the growth environment of cells in organisms. The purpose of high-density cell culture and high-concentration product expression is realized.

How does the CELLine bioreactor work?

Culture room
The medium chamber can store a large amount of medium. The volume of this layer is about 70 times that of the cell layer, which is used to meet the needs of the cells for fresh medium during the cell culture process.

Upper metabolite dialysis membrane
The upper dialysis membrane allows nutrients with a molecular weight of less than 10 kDa to permeate from the medium compartment to the cell compartment, and metabolic inhibitors to permeate from the cell compartment to the medium compartment, and ensures that the target protein remains in the cell compartment.

Cell compartment
The cell chamber provides an ideal environment for inoculating and collecting high-density cultures. This compartment is enriched for high density cells and their products of interest.

Lower gas dialysis membrane

In static culture, the rate of gas transfer will limit the density of the cell culture. The cells in the CELLine culture flask can directly obtain oxygen from the gas dialysis membrane at the bottom to balance oxygen and carbon dioxide and increase the gas transfer rate.

Seeding density and collection density: CELLine 350
Pre-culture (living cells): 8×106
Inoculation volume (mL): 5
Inoculum concentration (viable cells/mL): 1.5x106
Collection concentration (viable cells/mL): 107-108
Potency (mg/mL): 1-5
Monthly antibody yield (mg): 10-100
Specification:
Suspension culture, medium layer volume 1000mL, cell layer volume 15mL
Adherent culture, medium layer volume 1000mL, cell layer volume 15mL
Suspension culture, medium layer volume 350mL, cell layer volume 5mL
One CELLine350 reactor One bottle of CHO medium (1L/bottle)
One CELLine1000 reactor Three bottles of CHO medium (1L/bottle)
A bottle of culture medium (1L/bottle)

High-density cell culture The cell concentration in the cell chamber of CELLine is usually 107-108/mL, which is two orders of magnitude higher than that of ordinary static cell culture technology.

High product concentration
Thanks to the high cell density, the product concentration of cells cultured using CELLine is 1-5mg/mL, which is 50-100 times higher than that of traditional culture methods

Long-term continuous culture
CELLine's two-chamber culture technology. The user can harvest the product in a cycle and replace the culture medium. To achieve the purpose of long-term continuous cultivation.

Easy to operate
CELLine micro cell factory, easy to operate, no need to adjust the original culture system, you can conveniently use CELLine for culture

Reduce downstream processing
The two-chamber culture technology increases the cell concentration and product concentration by about two orders of magnitude compared with ordinary culture, and the concentration of enrichment culture itself can even be directly followed by subsequent operations without going through the complicated separation operations of ordinary culture.

Cost-effective
The unit output is high, and the use of CELLine can save a lot of cost compared with traditional T-flasks and multi-layer culture plates.

Shinetek CELLine 1000 Bioreactor, Shinetek Instruments Research Institute (CAT#-STEM-CC-3262-LGZ)