This dextran size-exclusion medium is designed for protein purification, molecular-weight determination, equilibrium-constant studies, and cell separation. It consists of dextran crosslinked through ether bonds to form a porous gel network. Molecular components are separated according to their ability to enter the internal pores. Large molecules are excluded and move through the column more rapidly. Intermediate molecules enter some of the pore volume and elute later. Smaller molecules access more of the matrix and experience greater retention. The 40-120 µm particles support general preparative operation. The stated fractionation range is 4000-100000 Da.
Specification
Model: Seplife G100 M Appearance: White spherical particles, odorless and tasteless Dry particle size: 40-120 µm Swelling volume: 15-20 mL/g dry powder Fractionation range: 4000-100000 Da Pressure behavior: Follows Darcy's law Flow behavior: Follows Darcy's law pH stability: 2-10 Applications: Protein purification, molecular-weight determination, equilibrium-constant measurement, and cell separation
Features
Preparative particle range 4000-100000 Da fractionation Suitable for protein purification Supports cell separation Equilibrium-study capability Stable dextran network High swelling volume Molecular-weight measurement