Seplife 6B Size-Exclusion Medium, 10000-4×10⁶ Da (CAT#: STEM-BC-3175-ZJF)

This agarose gel-filtration medium is designed for separation of biomolecules with substantial molecular-size differences. It uses six-percent crosslinked agarose gel particles. Molecules are separated according to their access to the internal pore structure. Large molecules are excluded from more pores and elute earlier. Smaller molecules enter a greater pore volume and elute later. The medium provides low nonspecific adsorption and high product recovery. It can be reused for repeated gel-filtration operations. The product is suitable where very high chromatographic resolution is not required. It is applicable to proteins, nucleic acids, and peptides in downstream bioprocessing.

Model: Seplife 6B
Source specification product grade: Seplife 4B
Appearance: White spherical gel, odorless and tasteless
Matrix: 6% crosslinked agarose gel
Globular-protein exclusion range: 10000-4×10⁶ Da
Particle size: 45-165 µm
Pressure-flow range: 180-300 cm/h at 0.1 MPa
Long-term pH stability: 4-9
Short-term CIP pH stability: 3-11
Chemical compatibility: Common gel-filtration solutions, 8 mol/L urea, and 6 mol/L guanidine hydrochloride
Heat resistance: 121°C in pH 7 water for 30 min

Size-exclusion separation
Broad molecular-weight range
Low nonspecific adsorption
High product recovery
Reusable agarose medium
Denaturant-compatible operation
Steam-treatment tolerance
Suitable for low-resolution fractionation

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